Pna conjugate for the treatment of diseases associated with hiv

ABSTRACT

The invention relates to peptide nucleic acid (PNA) conjugates which can be used for treating diseases correlated with HIV, wherein the peptide nucleic acid (PNA) inhibits the gene expression of HIV. The conjugates comprise the following components: (a) a transport mediator for the cell membrane, (b) an address protein or peptide for the import into the cell nucleus, and (c) a peptide nucleic acid (PNA) which is to be transported and can be hybridized with an HIV gene and can inhibit the expression of the HIV gene.

[0001] The present invention relates to peptide nucleic acid (PNA) conjugates which can be used for treating diseases correlated with HIV, the peptide nucleic acid (PNA) inhibiting the gene expression of HIV.

[0002] The incidence of the human immunodeficiency virus (HIV) is increasing world-wide in spite of the previous intensive research efforts made as to the development of effective treatment methods. HIV is counted among the lentiviral group of retroviruses and is one of the most intensively studied viruses. The HIV infection cycle starts with binding viral particles to the cell membrane of the target cells by means of a viral coat protein gp120/gp41. The virus initially binds to the CD4 protein, followed by binding to the obligatory co-receptor which is a member of the chemokin-receptor family. Main objectives of the HIV infection are T-helper cells and macrophages. Here, the viral core complex penetrates the cell and the virus is integrated into the viral genome in several steps (reverse transcription, introduction into the cell nucleus, integration into the chromosomes of the host cells as a DNA double strand). From this point of time, HIV is a permanent component of the cellular genome and can be considered an acquired genetic disease. HIV cannot replicate in the CD4+cell and for the “priming” of its promoters in the regulatory region (LTR) it requires cellular transcription factor for the transcription of early regulatory mRNAs which code for Tat, Rev and Nef proteins. The transactivator protein Tat is of special significance in the early phase of HIV-RNA synthesis. The Tat protein concentration correlates directly with the HIV-RNA amount. The interaction between Tat and TAR can also result in a strongly increased trans-activation of the viral gene expression by inducing the initiation of transcription as well as elongation.

[0003] Previous therapy approaches which aimed at a causal treatment have not brought about a decisive breakthrough in combating infections. Drug therapies have not yet been able to either stop HIV infections or heal diseases caused by them. Immunological strategies, e.g. inoculations, have not yet been successful on account of the high variability of the expression patterns of the HIV virus coat proteins and it seems that they will not be very promising in the future either. Another theoretical therapy approach is based on a molecular virus inactivation, e.g. via antisense RNAs for blocking viral nucleic acids. Although this therapy approach offers itself particularly for HIV, it appears to be highly problematic on account of the transient control of the HIV infection cycle and the presently almost unknown viral expression pattern. It also seems that a HIV proliferation control by ribozymes is very difficult to realize because special suitable CUG sequences of the virus genome have to be identified for such a strategy (since the ribozymes only cleave at this sequence motif), which appears to be extremely difficult, above all with respect to the very high HIV mutation rate.

[0004] The problem of an effective introduction of the antisense or ribozyme molecules into the target location also arises in connection with all of the above discussed procedures. The vectors previously used for this purpose, e.g. adeno-associated viruses (AAVs) have numerous drawbacks. AAVs are small parvoviruses having a single-stranded DNA. Their potential is their ability of infecting both dividing and non-dividing cells and penetrating the host genome. However, their major drawback is the lack of synthesis of sufficient amounts and lack of stability as a vector for hematopoietic cells. Vectors based on MLV (murine leukemia virus) have also been tested in numerous clinical studies. Although they seem to be non-toxic and theoretically suited as possible carriers for antisense constructs, they have the drawback that only very low titers are achieved in the host. Finally, vectors based on LV (lentiviruses) might also come into consideration, however, their major drawback is that they can only infect non-proliferating cells. Although this property would permit the superinfection of HIV-infected cells, they are nevertheless unsuited on account of their natural viral tropism.

[0005] The present invention is thus based on the technical problem of providing products which permit a specific and efficient therapy based on an inhibition of the HIV gene expression.

[0006] This technical problem is solved by providing the embodiments characterized in the claims.

[0007] In order to obtain a solution to the technical problem, the inventors developed a conjugate comprising the following components:

[0008] a transport mediator for the cell membrane (“P”),

[0009] an address protein or peptide (“AP”) for the import into the cell nucleus, and

[0010] a peptide nucleic acid which is to be transported and can be hybridized with a HIV gene and inhibits the expression thereof (“PNA”).

[0011] This modular conjugate has two decisive advantages:

[0012] (a) An efficient and site-directed PNA transport to the target location and thus a gene therapy is enabled by means of the components “P” and “AP”. These components do not only permit a rapid and effective transport of macromolecules such as PNA through cell membranes of living cells into the cytoplasm but, following a cytoplasmic activation of address peptide sequences, also an efficient transport into the cell nucleus.

[0013] (b) The use of the protease-resistant and nuclease-resistant peptide nucleic acids (PNAs) which are oligonucleotide derivatives whose sugar phosphate backbone is preferably substituted by ethyl-amine-linked α-amino-ethyl-glycine units permits a stable and efficient blocking of the transcription of the desired gene under physiological conditions on account of their physicochemical properties. An anti-gene strategy based on the antisense principle is pursued by these PNAs. However, in this strategy, the target is not the mRNA but the gene itself, e.g. a viral DNA intermediate or the viral DNA integrated into the genomic host DNA. Here, the PNAs hybridize via the formation of a triple helix to the target DNA. The target region can be a transcribed region of the target DNA, on the one hand, or a regulatory region the blocking of which via the PNAs also inhibits the transcription, on the other hand.

[0014] Regarding methods as to the production of the individual components of the conjugates and their linkage reference is made to German patent application No. 199 33 492.7. The synthesis of PNAs is known to a person skilled in the art and also described in Nielsen et al., Science 254 (1991), 1497-1500, for example.

[0015] The structure of the conjugate according to the invention is preferably: P - AP −(HIV-PNA)

[0016] more preferably with a spacer (“SP”): P - AP - SP - (HIV-PNA)

[0017] The transport mediator for the cell membrane (abbreviated as “P” above) represents a peptide or protein which can overcome the plasma membrane. The length of this peptide or protein is not limited as long as it has the above property. Examples of “P” originate preferably from the penetratin family (Derossi et al., Trends Cell Biol. 8 (1988), pages 84-87) or are transportan or parts thereof (Pooga et al., The Faseb Journal 12 (1998), page 68 et seq.), those of the penetratin family being preferred. An example of “P” is a penetratin having the following sequence: NH₂-RQIKIWFQNRRMKWKK-

[0018] The select “P” sequence is produced biologically (purification of natural transport mediator proteins or cloning and expression of the sequence in a eukaryotic or prokaryotic expression system) and preferably synthetically, e.g. according to the Merrifield method (Merrifield, J. Am. Chem. Soc. 85 (1963), 2149).

[0019] For the selection of the address protein or peptide (abbreviated by “AP” above) the person skilled in the art can chose controlling peptides or polypeptides by means of the known amino acid sequences for the import into the cell nucleus. In principle, the length of this address peptide or protein is not limited as long as it has the property of ensuring a cell nucleus-specific transport. In general “APs”, which contain a cell nucleus-specific recognition signal and thus direct the PNAs into the cell nucleus, are generally selected for the introduction of the PNAs. Fundamentally, the mere address sequence is sufficient for a transport into the cell nucleus. However, it is also possible to select “APs” which have a cell nucleus-specific peptidase cleavage site. Most favorably, this cleavage site is within the signal sequence but may also be attached thereto by additional amino acids to ensure the cleavage of the address sequence after reaching the cell nucleus. The select “AP” sequence is produced biologically (purification of natural transport mediator proteins or cloning and expression of the sequence in a eukaryotic or prokaryotic expression system) and preferably synthetically, e.g. according to the Merrifield method (Merrifield, J. Am. Chem. Soc. 85 (1963), 2149). Examples of suitable address proteins or peptides are: -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val; and H₃N⁺-Pro-Lys-Lys-Lys-Arg-Lys-Val- (= nuclear localization sequence from SV40 T-antigen).

[0020] Furthermore, the conjugate can optionally contain a spacer (abbreviated by “SP” above) which is preferably located between the address protein/peptide and the peptide nucleic acid (PNA) to be transported. However, it may also be present additionally or alternatively between the transport mediator and the address protein. The spacer serves for eliminating or favorably influencing optionally existing steric interactions between the components. The spacer can be selected from e.g.: glycine, polylysine, polyethylene glycol (PEG), derivatives of polymethacrylic acid or polyvinyl pyrrolidone (PVP).

[0021] A redox cleavage site, e.g. -cysteine-S—S-cysteine-O—N—H, is preferably found between the transport mediator and the address protein/peptide. The bond forming between transport mediator and address protein is a redox coupling (mild cell-immanent linkage by means of DMSO; Rietsch and Beckwith, Annu. Rev. Genet. 32 (1998), 163-84):

[0022] Cysteine-SH SH-cysteine

cysteine-S—S-cystine

[0023] The peptide nucleic acid (PNA) permits the inhibition of the transcription of genes essential for HIV, e.g. in that it hybridizes with a gene region which is transcribed or a regulatory region, i.e. a region which is responsible for the activation of the expression of a certain gene or certain genes. Suitable genes and suitable regions can be identified by the person skilled in the art by means of the previously known HIV genes or the function thereof. The peptide nucleic acids preferably have a length of at least 18 bases, peptide nucleic acids with a length of at least 20 bases being particularly preferred. The peptide nucleic acid can also optionally be labeled, e.g. radioactively (e.g. linked with an alpha-, beta- or gamma radiator), with a dyestuff, with biotin/avidine, etc.

[0024] The conjugate constituents “P” and “AP” are synthesized preferably synthetically according to the Merrifield method (Merrifield, J. Am. Chem. Soc. 85 (1963), 2149). The other constituents (e.g. spacers and/or PNAs) are linked thereto by covalent chemical bond. The redox cleavage site is inserted between “P” and “AP” chemically by the above mentioned redox coupling. A covalent bond, preferably an acid amide bond, is also present between an optionally present spacer and the PNA or the address protein and the PNA. Possible alternatives are ether or ester bonds, depending on the functional group(s) existing in the substance to be conjugated.

[0025] In a particularly preferred embodiment of the conjugate according to the invention the peptide nucleic acid (PNA) hybridizes with the HIV-tat gene or HIV-rev gene. Based on the special viral HIV cycle the tat and rev genes are two preferred molecular targets for an anti-HIV therapy. The products of both genes act as essential regulatory proteins for trans-activating the HIV gene expression by binding to HIV-mRNA. Tat binds to TAR (“trans-activating-response-element”) near the HIV-RNA 5′ end and Rev interacts with RRE (“Rev-responsive-element”) of the env gene.

[0026] The genomic organization of HIV-1 is shown in FIG. 1.

[0027] The sequences coding for HIV-1 are published in Ratner et al., Nature 313, pp. 277-284 (1985).

[0028] In a particularly preferred embodiment of the conjugate according to the invention, the PNAs hybridize with sequences of the HIV-1 LTR region (FIG. 2). Advantageous PNAs comprise the below sequences: TTATTTCCTCTTTTGTTG ATTAC*TAC*GTC*TC*TC*C*GTT TATC*GGTTTTTAAC*GTC*C*C* TC*C*TTTTC*C*C*C*GAC*AAC*C*TTTAC*

[0029] The use of pseudoisocytosine has the advantage that pH-independent hybridization is possible.

[0030] In another preferred embodiment, the PNAs are directed against the polypurine tract, central DNA flap, Nef or NCp7 (Vpr, Vpu [see FIG. 7]. For this purpose, the inventors conducted various experiments which are shown in FIGS. 9 to 13.

[0031] Preferred PNAs against the above-mentioned regions are as follows:

[0032] Sequences HIV-1 (Reference is made Herein to FIGS. 7-13)

[0033] Abbreviations:

[0034] L=linker

[0035] J=pseudoisocytosine or cytosine For HIV: c-PPT and 3′-PPT 4821-36/9116-31 (general sequence): PNA I (Polypurine tract) N-TCC CCC CTT TTC TTT T-L-TTT TJT T c-PPt target: DNA (+) or RNA 4821-39 N-CA ATC CCC CCT TTT CTT T-L-TT TJT TT PNA Ia 1) nbIaNLS+ 2) nbIaNLS− N-CA ATC CCC CCT TTT CTT T PNA Ib (the same sequence without linker part) 3) nbIbNLS+ 4) nbIbNLS− DNA (−) 4800-20 N-GTA TTC ATC CAC AAT TTT PNA II 5) nbIIaNLS+ 6) nbIIbNLS− DNA (+) 4800-20 N-AAA TTG TGG ATG AAT ACT PNA III 7) nbIIIaNLS+ 8) nbIIIbNLS− Flap: PNA IV DNA(−) 4861-80 N-TAG TAG ACA TAA TAG CAA PNA IVa 9) nbIVaNLS− Another DNA (−) between c-PPT and Tar to shorten the “flap” length: DNA (+) 4841-60 N-TCC CCT GCA CTG TAC CCC PNA IVb 10) nbVbNLS− Nef: DNA (−) 9095-9115 N-AGA TCT TAG CCA CTT TTT-C PNA Va 11) nbVaNLS+ 9136-56 N-GGC TAA TTC ACT CCC AAC-C PNA Vb 12) nbVbNLS+ IN site (3′) 9746-66: N-TAG AGA TTT TCC ACA CTG PNA Vc 13) nbVcNLS+ Seq is directed against the start of gag: N-cac cca tct ctc tcc ttc (no linker) PNA VI 14) nbVINLS− Splice acceptor site: N-jtt jtt-L-ttc ttc ctg cca tag PNA VII 15) nbXNLS+ 16) nbXNLS− TAR: N-cag gct caa atc tgg tct-L-tjt PNA VII 17) nbXNLS− NCp7: N-ATT ACT ACG TCT CTC CGT (not tested) PNA VIII N-TAT CGG TTT TTA ACG TCC 18) smVIIIaNLS+ N T TTT JJT-linker-TCC TTT TCC CCG ACA ACC 19) smVIIIaNLS+ 20) smVIIIcNLS+ Random sequence as a control: N-CAT ACT TGA CTC GTT ATC-C PNA IX N-CAT ACT TGA CTC GTT ATC-C 21) IX NLS− 22) IX NLS+ BDV PNA: (This sequence was tested for BDV spreading; FIG. 8) N-TCC CTA CGC CGC CTT CTC-C terminus

[0036] In another preferred embodiment the PNAs (e.g. PNA VI above) are directed against the viral RNA, the molecular target representing the Gag-splice acceptor site.

[0037] Finally, the present invention also relates to a medicament containing a conjugate according to the invention, optionally together with a suitable carrier, and to the use thereof for an HIV therapy. In this connection, in particular the parenteral or intravenous application has proved suited.

[0038] The invention is further described by means of the figures wherein:

[0039]FIG. 1 shows the genomic organization of HIV-1

[0040]FIG. 2 shows sequences of the HIV-1LTR region

[0041]FIG. 3 shows a plasmid map of pEGFP-C3

[0042]FIG. 4 shows viral sites of attack of the pseudo-isocytosine-containing PNAs

[0043]FIG. 5 shows CLSM pictures (non-activated control in HeLa)

[0044]FIG. 6 shows CLSM pictures (after activation in HeLa)

[0045]FIG. 7 shows PNA targets

[0046]FIG. 8 shows studies of BDV (Borna disease virus; retrovirus) instead of HIV to prove that other retroviruses can also be inhibited by PNA constructs

[0047]FIG. 9 shows the efficiency of PNA in the early phase of viral infection The biological effect was measured by transactivation of a reporter gene construct (LacZ)

[0048]FIG. 10 shows the results of the reporter gene assay after treatment with different PNAs. Significant results were observed with PNAs against cPPT (polypurine tract)

[0049]FIG. 11 shows the use of PNAs in the early and late phases of viral infection; testing by ELISA against p24 virus protein. Different PNAs against cPPT/flap/Nef/gag/random were capable of drastically reducing the viral p24

[0050]FIG. 12 shows the reporter gene assay with anti-FLAP PNA combined with other PNAs. The combination of PNA[IVb]+PNA[IIINLS]+PNA[Vb] (500 nM concentration) resulted in an optimum β-Gal reduction.

[0051]FIG. 13 shows that chronically HIV-infected cells were studied by viral p24 ELISA following PNA treatment (72 and 144 h after the PNA application). The best effect was obtained with cPPT (polypurine tract) by PNA[IINLS].

[0052] The invention is further described by means of the below examples.

EXAMPLE 1

[0053] The HIV-1 “long terminal repeat” (LTR) codes for the transcription promoter. Sequence analyses prove the existence of a single LTR enhancer promoter configuration for all of the presently studied HIV-1 subtypes. Transcription studies using EGF reporter constructs show its functionality.

[0054] LTR-EGF Construct:

[0055] Based on biocomputing data, the sequence AC S72615, which relates to the HIV subtype HIV4B6 was selected representatively using the HUSAR program of DKFZ, the SRS (sequence Retrieval System) and the multiple alignment algorithm (MALIGN) (Shiramizu et al., Cancer Res. 65, pp. 2069-2072 (1994)). HIV4B6-LTR: CCAATAAAGG AGAAAACAAC TGCTTGTTAC ACCC⁽⁻¹⁸⁾TATAAG CCAGCATAAA GC₍₊₁₎ ATG GA

[0056] The Ase I (8/−52)-Nhe I (64/+1)-LTR fragment was synthesized according to the known phosphoramidite method and cloned into a pEGFP-C3/Variant (without PCMV) (FIG. 3) (Clontech company, Heidelberg).

[0057] Cloned Fragment:

[0058] Then, the pasmid DNA was replicated in E. coli in LB-Amp culture medium under ampicillin selection pressure. The isolation and preparation of the plasmid DNA were carried out with the Mini-Prep-DNA kit (Clontech company) according to the manufacturer's instructions.

[0059] Cell Culture and Transfection Assay:

[0060] The human cervical carcinoma suspension cell line HeLa-S (tumor bank DKFZ) was used. The cells were cultured in MEM″Joklik″ (Minimal Essential Medium; Sigma company) with 10% FCS (Sigma company), glutamine (Gibco company) in a CO₂ atmosphere at 37° C.

[0061] The following shuttle construct was produced:

[0062] This construct was transfected into the HeLa cells according to the Britten and Kohne protocol (Science, Vol. 161, No. 3841, pp. 529-540, 1986). HeLa-S is transfected by direct addition of the shuttle construct to the culture medium at a concentration of 100 pM at 37° C. for 3 hours under incubation in 5% CO₂ atmosphere. Then, the hybridization was carried out with the LTR-EGF construct analogously to the above mentioned Britten and Kohne protocol. The following complex forms:

[0063] The HeLa-S cells are cultured in 8-chamber glass plates in Petri dishes under 5% CO₂ at 370 for 48 hours.

[0064] In order to activate the plasmid, it is necessary to separate the shuttle system from the plasmid DNA. For this purpose, the plates are heated to 45° C. in a water bath for 1 minute. Having changed the medium, the HeLa-S cells are further cultured. The transcription determination of GFP under LTR control is effected by means of fluorescence reader analysis after 48, 72 and 96 hours (excitation: 488 nm; emission: 520 nm). GFP is localized by means of confocal laser scanning microscopy (CLSM) analogously but in 8-chamber glass plates in Petri dishes under 5% CO₂ at 37° C. for 48, 72 and 96 hours (excitation: 488 nm; emission: 520 nm). The results are shown in FIGS. 5 and 6.

EXAMPLE 2

[0065] Here, a method is described which permits to determine a PNA effect in an early infection phase of HIV-1. Reference is made to FIGS. 9-11.

[0066] HeLa cells which were transfected with a stably expressed LacZ reporter gene were used as a model. The test was carried out in a 96-well plate. HeLa cells are suspended in RPMI medium and plated out. The cell number was 20000 cells per well. The pipetting volume is 100 μl/well. 18 different PNA sequences (see FIG. 10) plus one control were tested (X=further viral control (e.g. BDV)). The PNA construct concentrations were 10 pM, 100 pM and 1 nM. The PNA constructs were produced in analogy with Example 1. After one hour, the viral infection was effected. The viral effect on the LTR promoter was determined via LacZ activity by means of a photometer.

1 54 1 16 PRT Homo sapiens 1 Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 1 5 10 15 2 8 PRT Artificial Sequence Addressprotein 2 Pro Pro Lys Lys Lys Arg Lys Val 1 5 3 7 PRT Artificial Sequence Addressprotein 3 Pro Lys Lys Lys Arg Lys Val 1 5 4 18 DNA Artificial Sequence PNA 4 ttatttcctc ttttgttg 18 5 19 DNA Artificial Sequence PNA 5 attactacgt ctctccgtt 19 6 19 DNA Artificial Sequence PNA 6 tatcggtttt taacgtccc 19 7 23 DNA Artificial Sequence PNA 7 tccttttccc cgacaacctt tac 23 8 23 DNA Artificial Sequence PNA I 8 tccccccttt tctttttttt ctt 23 9 25 DNA Artificial Sequence PNA Ia 9 caatcccccc ttttcttttt tcttt 25 10 18 DNA Artificial Sequence PNA Ib 10 caatcccccc ttttcttt 18 11 18 DNA Artificial Sequence PNA II 11 gtattcatcc acaatttt 18 12 18 DNA Artificial Sequence PNA III 12 aaattgtgga tgaatact 18 13 18 DNA Artificial Sequence PNA IV 13 tagtagacat aatagcaa 18 14 18 DNA Artificial Sequence PNA IVb 14 tcccctgcac tgtacccc 18 15 18 DNA Artificial Sequence PNA Va 15 agatcttagc cacttttt 18 16 18 DNA Artificial Sequence PNA Vb 16 ggctaattca ctcccaac 18 17 18 DNA Artificial Sequence PNA Vc 17 tagagatttt ccacactg 18 18 18 DNA Artificial Sequence PNA VI 18 cacccatctc tctccttc 18 19 21 DNA Artificial Sequence PNA VII 19 caggctcaaa tctggtcttc t 21 20 18 DNA Artificial Sequence PNA VIII 20 attactacgt ctctccgt 18 21 18 DNA Artificial Sequence PNA VIII 21 tatcggtttt taacgtcc 18 22 25 DNA Artificial Sequence PNA VIII 22 ttttccttcc ttttccccga caacc 25 23 18 DNA Artificial Sequence PNA IX 23 catacttgac tcgttatc 18 24 18 DNA Artificial Sequence PNA IX 24 catacttgac tcgttatc 18 25 18 DNA Artificial Sequence BDV PNA 25 tccctacgcc gccttctc 18 26 57 DNA Human immunodeficiency virus 26 ccaataaagg agaaaacaac tgcttgttac accctataag ccagcataaa gcatgga 57 27 53 DNA Artificial Sequence Cloned fragment 27 ccaataaagg agaaaacaac tgccttgtta caccctataa gccagcataa agc 53 28 18 DNA Artificial Sequence Shuttle-construct 28 ttatttcctc ctttgttg 18 29 9 DNA Artificial Sequence Shuttle-construct 29 ttatttcct 9 30 2460 DNA Human immunodeficiency virus 30 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca 60 cacaaggcta cttccctgat tagcagaact acacaccagg gccagggatc agatatccac 120 tgacctttgg atggtgctac aagctagtac cagttgagcc agagaagtta gaagaagcca 180 acaaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgacccgg 240 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag 300 agctgcatcc ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg 360 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 420 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag 660 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 780 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgatgggaa 840 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca 900 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt 960 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca 1020 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc 1080 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa 1140 gcagcagctg acacaggaca cagcaatcag gtcagccaaa attaccctat agtgcagaac 1200 atccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa 1260 gtagtagaag agaaggcttt cagcccagaa gtgataccca tgttttcagc attatcagaa 1320 ggagccaccc cacaagattt aaacaccatg ctaaacacag tggggggaca tcaagcagcc 1380 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag agtgcatcca 1440 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca 1500 ggaactacta gtacccttca ggaacaaata ggatggatga caaataatcc acctatccca 1560 gtaggagaaa tttataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat 1620 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta 1680 gaccggttct ataaaactct aagagccgag caagcttcac aggaggtaaa aaattggatg 1740 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg 1800 ggaccagcgg ctacactaga agaaatgatg acagcatgtc agggagtagg aggacccggc 1860 cataaggcaa gagttttggc tgaagcaatg agccaagtaa caaattcagc taccataatg 1920 atgcagagag gcaattttag gaaccaaaga aagattgtta agtgtttcaa ttgtggcaaa 1980 gaagggcaca cagccagaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga 2040 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc 2100 tggccttcct acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc 2160 ccaccagaag agagcttcag gtctggggta gagacaacaa ctccccctca gaagcaggag 2220 ccgatagaca aggaactgta tcctttaact tccctcaggt cactctttgg caacgacccc 2280 tcgtcacaat aaagataggg gggcaactaa aggaagctct attagataca ggagcagatg 2340 atacagtatt agaagaaatg agtttgccag gaagatggaa accaaaaatg atagggggaa 2400 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgtggacata 2460 31 181 DNA Human immunodeficiency virus misc_feature (123)..(123) n is a, c, g, or t 31 gcctggccat aaagcaagaa ttttggctga ggcaatgagc caggtaacaa atacrgctgt 60 aatgatgcag cgaaacaact ttaagggtca aagaaaaatt attaaatgtt tcaactgtgg 120 canggaggga cytagcaaaa aattgcaggg cccctaggdd gddgggttgt tggaaatgta 180 a 181 32 213 DNA Human immunodeficiency virus 32 catgtcgggg agtgggagga cctagccaca aagccagagt gttggctgag gcaatgagcc 60 aagcaaataa tacaaacata atgatgcaga gaaacaactt taaaggccct aaaagaatta 120 ttaaatgttt caactgtggc aaggaagggc acttagccag aaattgcagg gcccctagga 180 aaaaaggctg ttggaaatgt ggaaaggaag gac 213 33 202 DNA Human immunodeficiency virus misc_feature (140)..(142) n is a, c, g, or t 33 catgtcasgg agtgggggac ccggccataa agcaagagtt ttggctgaag caatgagcca 60 agtaacacca ccagctaaca taatgatgca gagaggcaat tttaggaacc aaagaaagac 120 tgttaagtgt ttcaattgtn nndaagaagg gcayatagcc aaaaattgca gggcccctag 180 gaadaagggc tgttggaaat gt 202 34 192 DNA Human immunodeficiency virus misc_feature (6)..(6) n is a, c, g, or t 34 cataangcaa gagttttggc tgaagcaatg agccaagtaa cacaaccagc taccataatg 60 atgcagagag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgbbbvaaa 120 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtggt 180 agggaaggac ac 192 35 206 DNA Human immunodeficiency virus 35 agtgggaggm cccggccama aagcaagggt tttggcggaa gcaatgagcc aagtaacaaa 60 ttcacctgcc ataatgatgc agagaggcaa ttttaggaac caaagaaaaa ctgttaagtg 120 tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta ggaaaagggg 180 ctgttggaaa tgtgghaagg aaggam 206 36 206 DNA Human immunodeficiency virus 36 agtgggaggm cccggccama aagcaagggt tttggcggaa gcaatgagcc aagtaacaaa 60 ttcacctgcc ataatgatgc agagaggcaa ttttaggaac caaagaaaaa ctgttaagtg 120 tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta ggaaaagggg 180 ctgttggaaa tgtgghaagg aaggam 206 37 60 DNA Human immunodeficiency virus misc_feature (19)..(21) n is a, c, g, or t 37 aggtaacaaa tacrgctgnn ntaatgatgc agcgaaacaa ctttaagggt nncaaagaaa 60 38 60 DNA Human immunodeficiency virus misc_feature (15)..(17) n is a, c, g, or t 38 aagcaaataa tacannnaac ataatgatgc agagaaacaa ctttaaaggc ncctaanaag 60 39 60 DNA Human immunodeficiency virus misc_feature (46)..(47) n is a, c, g, or t 39 aagtaacacc accagctaac ataatgatgc agagaggcaa ttttanngga accaaagaaa 60 40 60 DNA Human immunodeficiency virus misc_feature (46)..(47) n is a, c, g, or t 40 aagtaacaca accagctacc ataatgatgc agagaggcaa ttttanngga accaaagaaa 60 41 60 DNA Human immunodeficiency virus misc_feature (46)..(47) n is a, c, g, or t 41 aagtaacaaa ttcacctgcc ataatgatgc agagaggcaa ttttanngga accaaagaaa 60 42 60 DNA Human immunodeficiency virus misc_feature (46)..(47) n is a, c, g, or t 42 aagtaacaaa ttcacctgcc ataatgatgc agagaggcaa ttttanngga accaaagaaa 60 43 60 DNA Human immunodeficiency virus misc_feature (27)..(27) n is a, c, g, or t 43 aattattaaa tgtttcaact gtggcangga gggacacyta gcaaaaaatt gcagggcccc 60 44 60 DNA Human immunodeficiency virus 44 aattattaaa tgtttcaact gtggcaagga agggcactta gccagaaatt gcagggcccc 60 45 60 DNA Human immunodeficiency virus misc_feature (23)..(25) n is a, c, g, or t 45 gactgttaag tgtttcaatt gtnnndaaga agggcayata gccaaaaatt gcagggcccc 60 46 60 DNA Human immunodeficiency virus 46 gactgttaag tgtttcaatt gbbbvaaaga agggcacata gccaaaaatt gcagggcccc 60 47 60 DNA Human immunodeficiency virus 47 aactgttaag tgtttcaatt gtggcaaaga agggcacata gccaaaaatt gcagggcccc 60 48 60 DNA Human immunodeficiency virus 48 aactgttaag tgtttcaatt gtggcaaaga agggcacata gccaaaaatt gcagggcccc 60 49 30 DNA Human immunodeficiency virus misc_feature (26)..(28) n is a, c, g, or t 49 taggddgddg ggttgttgga aatgtnnnaa 30 50 38 DNA Human immunodeficiency virus 50 taggaaaaaa ggctgttgga aatgtggaaa ggaaggac 38 51 25 DNA Human immunodeficiency virus 51 taggaadaag ggctgttgga aatgt 25 52 40 DNA Human immunodeficiency virus 52 taggaaaaag ggctgttgga aatgtggtag ggaaggacac 40 53 38 DNA Human immunodeficiency virus 53 taggaaaagg ggctgttgga aatgtgghaa ggaaggam 38 54 38 DNA Human immunodeficiency virus 54 taggaaaagg ggctgttgga aatgtgghaa ggaaggam 38 

1. A conjugate for mediating a cell nucleus-specific transport of a peptide nucleic acid (PNA) which can be hybridized to a transcribed HIV gene or a HIV gene involved in the regulation of gene expression or a part thereof, wherein the conjugate comprises the following components: a transport mediator for the cell membrane, an address protein or peptide for the import into the cell nucleus, and a peptide nucleic acid (PNA) which is to be transported and can be hybridized with an HIV gene.
 2. The conjugate according to claim 1, wherein the transport mediator is a peptide or protein which can overcome the plasma membrane.
 3. The conjugate according to claim 1, wherein the transport mediator originates from the penetratin family or is transportan or parts thereof.
 4. The conjugate according to claim 3, wherein one of the penetratins has the following sequence: NH₂—RQIKIWFQNRRMKWKK—
 5. The conjugate according to claim 1, wherein the address protein or peptide is selected from: -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val; and H₃N+-Pro-Lys-Lys-Lys-Arg-Lys-Val- (= nuclear localization sequence from SV40-T antigen).


6. The conjugate according to claim 1, wherein the conjugate has the following structure: transport mediator-address protein-peptide nucleic acid (PNA).
 7. The conjugate according to claim 1, wherein a spacer is also present, where appropriate.
 8. The conjugate according to claim 7, wherein the spacer is located between the address protein and the peptide nucleic acid (PNA).
 9. The conjugate according to claim 8, wherein the spacer is polylysine, polyethylene glycol or polyvinyl pyrrolidone.
 10. The conjugate according to claim 1, wherein the peptide nucleic acid hybridizes with the HIV-tat gene or HIV-rev gene or a part thereof.
 11. The conjugate according to claim 10, wherein the peptide nucleic acid comprises a sequence selected from the following group: TTATTTCCTCTTTTGTTG ATTAC*TAC*GTC*TC*TC*C*GTT TATC*GGTTTTTAAC*GTC*C*C* TC*C*TTTTC*C*C*C*GAC*AAC*C*TTTAC* (with; C* = pseudoisocytosine)


12. The conjugate according to claim 1, wherein the peptide nucleic acid is directed against the polypurine tract, central DNA flap, Nef, NCp7; or the Gag-splicing site on the RNA level.
 13. A medicament containing a conjugate according to claim
 1. 14. A method for reducing or treating HIV infection, the method comprising administering an effective amount of the conjugate of claim 1 for HIV therapy.
 15. The conjugate according to claim 3, wherein the address protein or peptide is selected from: -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val; and H₃N+-Pro-Lys-Lys-Lys-Arg-Lys-Val- (= nuclear localization sequence from SV40-T antigen).


16. The conjugate according to claim 4, wherein the address protein or peptide is selected from: -Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val; and H₃N+-Pro-Lys-Lys-Lys-Arg-Lys-Val- (= nuclear localization sequence from SV40-T antigen).


17. The conjugate according to claim 1, wherein the peptide nucleic acid comprises a sequence selected from the following group: TTATTTCCTCTTTTGTTG ATTAC*TAC*GTC*TC*TC*C*GTT TATC*GGTTTTTAAC*GTC*C*C* TC*C*TTTTC*C*C*C*GAC*AAC*C*TTTAC* (with; C = pseudoisocytosine)


18. The conjugate according to claim 16, wherein the peptide nucleic acid comprises a sequence selected from the following group: TTATTTCCTCTTTTGTTG ATTAC*TAC*GTC*TC*TC*C*GTT TATC*GGTTTTTAAC*GTC*C*C* TC*C*TTTTC*C*C*C*GAC*AAC*C*TTTAC*


19. The conjugate according to claim 4, wherein the peptide nucleic acid is directed against the polypurine tract, central DNA flap, Nef, NCp7; or the Gag-splicing site on the RNA level.
 20. The conjugate according to claim 16, wherein the peptide nucleic acid is directed against the polypurine tract, central DNA flap, Nef, NCp7; or the Gag-splicing site on the RNA level.
 21. A medicament containing a conjugate according to claim
 20. 22. A medicament containing a conjugate according to claim
 17. 23. A medicament containing a conjugate according to claim
 18. 24. The conjugate according to claim 16, wherein the conjugate has the following structure: transport mediator-address protein-peptide nucleic acid (PNA).
 25. The conjugate according to claim 4, wherein the peptide nucleic acid is protease-resistant and nuclease-resistant.
 26. The conjugate according to claim 1, wherein the peptide nucleic acid is protease-resistant and nuclease-resistant.
 27. The conjugate according to claim 1, wherein the peptide nucleic acid has a sugar phosphate backbone substituted by ethyl-amine-linked α-amino-ethyl-glycine units.
 28. The conjugate according to claim 16, wherein the peptide nucleic acid has a sugar phosphate backbone substituted by ethyl-amine-linked α-amino-ethyl-glycine units 